Room: Poster Area
Date: Thursday, 21 May 2026
Time: 16:15 - 17:15 CEST
Session code 3CV.8
New uses and better valorization of different biomass sources. Market transition towards a biobased future
Optimization of Trichosporon oleaginosus Cultivation Paving the Way Toward a Continuous two-stage Operation for Advanced Biofuel Feedstock Production
Short Introductive summary
This study aims to optimize the cultivation of the oleaginous yeast Trichosporon oleaginosus within an integrated heterotrophic–autotrophic system for producing lipid-rich biomass as a sustainable biofuel feedstock. The research focuses on refining parameters of both the growth and lipogenic phases to enable a continuous two-stage operation. Key factors investigated include nitrogen source selection, supplementation strategy, and dissolved oxygen (DO) control. Initial trials using different nitrogen sources identified an isonitrogenous ammonium sulfate–yeast extract mixture as the most efficient and cost-effective option. Subsequent bioreactor experiments tested three DO levels (10 %, 20 %, 30 %). Higher DO levels (20–30 %) promoted greater biomass accumulation (˜21 g L?¹) compared to 10 %, though lipid content remained under 20 % wt during growth. In the lipogenic phase, all conditions produced over 54 g L?¹ biomass with lipid content near 60 %. Ongoing experiments explore oxygen optimization between phases to improve lipid yield and energy efficiency. Continuous monitoring of nutrients, gases, and metabolites supports modeling and scaling toward a sustainable continuous operation
Presenter
Christos CHATZIDOUKAS
Aristotle University of Thessaloniki, Chemical Engineering Dpt., GREECE
Presenter's biography
Dr. Christos Chatzidoukas serves as an Assoc. Professor in the Department of Chemical Engineering at the Aristotle University of Thessaloniki. He is the Head of the Laboratory of Biochemical and Biotechnological Processes (LB2P). He obtained his doctoral degree from Imperial College, London in 2004.
Biographies and Short introductive summaries are supplied directly by presenters and are published here unedited
Co-authors:
A. Biziouras, Aristotle University of Thessaloniki, GREECE
G. Papapanagiotou, Aristotle University of Thessaloniki, GREECE
C. Samara, Aristotle University of Thessaloniki, GREECE
G. Amanatidis, Aristotle University of Thessaloniki, GREECE
E. Koula, Aristotle University of Thessaloniki, GREECE
P. Karakasis, Aristotle University of Thessaloniki, GREECE
C. Chatzidoukas, Aristotle University of Thessaloniki, GREECE
Session reference: 3CV.8.4